Effect of Overexpression of a Soluble Pyridine Nucleotide Transhydrogenase (UdhA) on the Production of Poly(3-hydroxybutyrate) in Escherichia coli

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• 作者：Ailen M. Sá ; nchez ; Jared Andrews ; Insiya Hussein ; George N. Bennett ; and Ka-Yiu San
• 刊名：Biotechnology Progress
• 出版年：2006
• 出版时间：April 2006
• 年：2006
• 卷：22
• 期：2
• 页码：420 - 425
• 全文大小：148K
• 年卷期：v.22,no.2(April 2006)
• ISSN：1520-6033

文摘

A soluble pyridine nucleotide transhydrogenase (UdhA) has been used to increase the productivityand yield of PHB in vivo. By inducing a high level of UdhA, which can transfer reducingequivalents between NAD and NADP, we have increased NADPH availability, resulting in highyield and productivity of PHB in Escherichia coli. Coexpression of the phb operon fromAlcaligenes eutrophus H16 and the native udhA from E. coli from high copy plasmids resultedin an increase in PHB yield from 49 to 66% g of PHB per gram of total cell dry weight and anincrease in final concentration from 3.52 to 6.42 g/L; the PHB concentration of the udhA carryingstrain is almost twice that of the control strain expressing only the phb operon. The results ofthis study demonstrate the effectiveness of cofactor manipulation and its application as a tool inmetabolic engineering.