Synthesis and Anticoagulant Activity of Polyureas Containing Sulfated Carbohydrates
详细信息 查看全文
- 作者：Yongshun Huang ; Maureen A. Shaw ; Eric S. Mullins ; Terence L. Kirley ; Neil Ayres
- 刊名：Biomacromolecules
- 出版年：2014
- 出版时间：December 8, 2014
- 年：2014
- 卷：15
- 期：12
- 页码：4455-4466
- 全文大小：642K
- ISSN：1526-4602
文摘
Polyurea-based synthetic glycopolymers containing sulfated glucose, mannose, glucosamine, or lactose as pendant groups have been synthesized by step-growth polymerization of hexamethylene diisocyanate and corresponding secondary diamines. The obtained polymers were characterized by gel permeation chromatography, nuclear magnetic resonance spectroscopy, and Fourier transform infrared spectroscopy. The nonsulfated polymers showed similar results to the commercially available biomaterial polyurethane TECOFLEX in a platelet adhesion assay. The average degree of sulfation after reaction with SO3 was calculated from elemental analysis and found to be between three and four 鈭扥SO3 groups per saccharide. The blood-compatibility of the synthetic polymers was measured using activated partial thromboplastin time, prothrombin time, thrombin time, anti-IIa, and anti-Xa assays. Activated partial thromboplastin time, prothrombin time, and thrombin time results indicated that the mannose and lactose based polymers had the highest anticoagulant activities among all the sulfated polymers. The mechanism of action of the polymers appears to be mediated via an anti-IIa pathway rather than an anti-Xa pathway.