In Vitro Assessment of Antibacterial Activity and Cytocompatibility of Silver-Containing PHBV Nanofibrous Scaffolds for Tissue Engineering

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• 作者：Zhi-Cai Xing ; Won-Pyo Chae ; Jin-Young Baek ; Moon-Jeong Choi ; Yongsoo Jung ; Inn-Kyu Kang
• 刊名：Biomacromolecules
• 出版年：2010
• 出版时间：May 10, 2010
• 年：2010
• 卷：11
• 期：5
• 页码：1248-1253
• 全文大小：412K
• 年卷期：v.11,no.5(May 10, 2010)
• ISSN：1526-4602

文摘

Infections with bacteria have become a serious problem in joint arthroplasty. This study reports about in vitro antibacterial activity and in vitro cell compatibility of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers loaded with metallic silver particles of a size of 5−13 nm. In vitro antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae was studied by microplate proliferation tests. The adhesion, viability, and proliferation properties of fibroblasts (NIH 3T3) and differentiation of osteoblasts (MC3T3-E1) were done to study in vitro cell compatibility of the scaffolds. As the results, only silver-containing PHBV nanofibrous scaffolds showed a high antibacterial activity and an inhibitory effect on the growth of both Staphylococcus aureus and Klebsiella pneumoniae bacteria. The nanofibrous scaffolds having silver nanoparticles <1.0% were free of in vitro cytotoxicity. To sum up, the PHBV nanofibrous scaffolds having nanoparticles <1.0 wt % showed not only good antibacterial activity but also good in vitro cell compatibility. It is considered that the PHBV nanofibrous scaffolds with silver nanoparticles <1.0 wt % have a potential to be used in joint arthroplasty.