Graphite-Coated Magnetic Nanoparticle Microarray for Few-Cells Enrichment and Detection
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- 作者：Zhuo Chen ; Guosong Hong ; Hailiang Wang ; Kevin Welsher ; Scott M. Tabakman ; Sarah P. Sherlock ; Joshua T. Robinson ; Yongye Liang ; Hongjie Dai
- 刊名：ACS Nano
- 出版年：2012
- 出版时间：February 28, 2012
- 年：2012
- 卷：6
- 期：2
- 页码：1094-1101
- 全文大小：514K
- 年卷期：v.6,no.2(February 28, 2012)
- ISSN：1936-086X
文摘
Graphite-coated, highly magnetic FeCo core鈥搒hell nanoparticles were synthesized by a chemical vapor deposition method and solubilized in aqueous solution through a unique polymer mixture modification, which significantly improved the biocompatibility and stability of the magnetic nanoparticles (MNPs). Such functionalized MNPs were proven to be very stable in different conditions which would be significant for biological applications. Cell staining, manipulation, enrichment, and detection were developed with these MNPs. Under external magnetic manipulation, the MNP-stained cells exhibited directed motions. Moreover, MNPs were printed on substrates to modulate the magnetic field distribution on the surface. Capture and detection of sparse populations of cancer cells spiked into whole blood has been explored in a microarray fashion. Cancer cells from hundreds down to only two were able to be simply and efficiently detected from 1 mL of whole blood on the MNP microarray chips. Interestingly, the cells captured through the MNP microarray still showed viability and adhered to the MNP spots after incubation, which could be utilized for cancer cell detection, localized growth, and proliferation.