Use of Abasic Site-Containing DNA Strands for Nucleobase Recognition in Water

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• 作者：Keitaro Yoshimoto ; Seiichi Nishizawa ; Masakazu Minagawa ; and Norio Teramae
• 刊名：Journal of the American Chemical Society
• 出版年：2003
• 出版时间：July 30, 2003
• 年：2003
• 卷：125
• 期：30
• 页码：8982 - 8983
• 全文大小：103K
• 年卷期：v.125,no.30(July 30, 2003)
• ISSN：1520-5126

文摘

Nucleobase recognition in water is successfully achieved by the use of an abasic site (AP site) as the molecular recognition field. We intentionally construct the AP site in DNA duplex so as to orient the AP site toward a target nucleobase and examine the complexation of 2-amino-7-methylnaphthyridine (AMND) with nucleobases at the AP site. AMND is found to selectively bind to cytosine (C) base with a 1:1 binding constant of >10⁶ M^-1, accompanied by remarkable quenching of its fluorescence. In addition to hydrogen bonding, a stacking interaction with nucleobases flanking the AP site seems responsible for the binding properties of AMND at the AP site. Possible use of AMND is also presented for selective and visible detection of a single-base alternation related to the cytosine base.