Adenosine-1,3-diazaphenoxazine Derivative for Selective Base Pair Formation with 8-Oxo-2鈥?deoxyguanosine in DNA

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• 作者：Yosuke Taniguchi ; Ryota Kawaguchi ; Shigeki Sasaki
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：May 18, 2011
• 年：2011
• 卷：133
• 期：19
• 页码：7272-7275
• 全文大小：818K
• 年卷期：v.133,no.19(May 18, 2011)
• ISSN：1520-5126

文摘

The selective detection of 8-oxo-2鈥?deoxyguanosine (8-oxo-dG) in DNA without chemical or enzymatic treatment is an attractive tool for genomic research. We designed and synthesized the non-natural nucleoside analogue, the adenosine-1,3-diazaphenoxazine (Adap) derivative, for selective recognition of 8-oxo-dG in DNA. This study clearly showed that Adap has a highly selective stabilizing effect on the duplex containing the Adap鈥?-oxo-dG base pair. Furthermore, the fluorescent property of Adap was shown to be useful for the selective detection of 8-oxo-dG in the duplex DNA. To the best of our knowledge, this is the first successful demonstration of a non-natural nucleoside with a high selectivity for 8-oxo-dG in DNA.