Simultaneous Quantification of Hg2+ and MeHg+ in Aqueous Media with a Single Fluorescent Probe by Multiplexing in the Time Domain

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• 作者：Ziqian Zhang ; Baoyan Zhang ; Xuhong Qian ; Zhong Li ; Zhiping Xu ; Youjun Yang
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：December 2, 2014
• 年：2014
• 卷：86
• 期：23
• 页码：11919-11924
• 全文大小：427K
• ISSN：1520-6882

文摘

Development of a molecular probe for selective detection of MeHg⁺ in the presence of Hg²⁺ is a mission impossible to accomplish. Speciation analysis of two substrates with a single kinetic trace exploiting their differential reactivity toward a single probe, i.e., multiplexing in the time domain, is a cost-effective and powerful alternative. We have developed such a probe (Hg410) for simultaneously quantification of Hg²⁺ and MeHg⁺ in aqueous media. Hg410 is designed via the 鈥渃ovalent-assembly鈥?approach, displays a zero background, and bears a very concise molecular construct. It has harnessed proximity-based catalysis to achieve high reactivity toward Hg²⁺ and MeHg⁺. An unprecedentedly low detection limit of ca. 4.6 pM and 160 pM was measured for Hg²⁺ and MeHg⁺, respectively.