文摘
An ion mobility-mass spectrometry technique that incorporates a differentially pumped orifice-skimmer cone(OSC) region at the exit of the drift tube has beendeveloped. The OSC region is similar in design to thoseused in electrospray ionization sources and offers improvements in ion transmission (by factors of ~5-10compared with previous designs) and the ability to inducefragmentation of mobility-separated ions. The separationof ions prior to dissociation at the skimmer cone allowsthe origin of fragment ions to be examined. Here, wedescribe the experimental design and demonstrate theapproach by examining fragment ions that are commonto multiple charge states and different gas-phase ionconformations of electrosprayed angiotensin II and[Sar1, Val5, Ala8] angiotensin II peptides.