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• 作者：Hyuk-Gu Park^† ; ^‡ ; ; Youn Young Shim*^§ ; ; Seung-Oh Choi^‡ ; ; Won-Mok Park*^† ;
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2009
• 出版时间：March 25, 2009
• 年：2009
• 卷：57
• 期：6
• 页码：2147-2154
• 全文大小：294K
• 年卷期：v.57,no.6(March 25, 2009)
• ISSN：1520-5118

文摘

Sparassis crispa and Phellinus linteus are edible/medicinal mushrooms that have remarkably high contents of β-(1→3)-d-glucan, which acts as a biological response modifier, but difficulty in cultivating the fruiting bodies and extraction of β-d-glucan have restricted detailed studies. Therefore, a novel process for nanoparticle extraction of Sparan, the β-d-glucan from Sparassis crispa, and Phellin, the β-d-glucan from Phellinus linteus, has been investigated using insoluble tungsten carbide as a model for nanoknife technology. This is the first report showing that the nanoknife method results in high yields of Sparan (70.2%) and Phellin (65.2%) with an average particle size of 150 and 390 nm, respectively. The extracted Sparan with β-(1→3) linkages showed a remarkably high water solubility of 90% even after 10 min of incubation at room temperature. Therefore, it is likely that this nanoknife method could be used to produce β-d-glucan for food, cosmetic, and pharmaceutical industries.