A Genetically Encoded Infrared Probe
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- 作者：Kathryn C. Schultz ; Lubica Supekova ; Youngha Ryu ; Jianming Xie ; Roshan Perera ; Peter G. Schultz
- 刊名：Journal of the American Chemical Society
- 出版年：2006
- 出版时间：November 1, 2006
- 年：2006
- 卷：128
- 期：43
- 页码：13984 - 13985
- 全文大小：76K
- 年卷期：v.128,no.43(November 1, 2006)
- ISSN：1520-5126
文摘
An orthogonal tRNA/aminoacyl-tRNA synthetase pair has been evolved that makes it possible to selectively and efficiently incorporate para-cyanophenylalanine (pCNPhe) into proteins in E. coli at sites specified by the amber nonsense codon, TAG. Substitution of pCNPhe for histidine-64 in myoglobin (Mb) affords a sensitive vibrational probe of ligand binding. This methodology provides a useful infrared reporter of protein structure, biomolecular interactions, and conformational changes.