Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection
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- 作者：Honglun Wang ; Lily M. L. Ou ; Yourui Suo ; Hua-Zhong Yu
- 刊名：Analytical Chemistry
- 出版年：2011
- 出版时间：March 1, 2011
- 年：2011
- 卷：83
- 期：5
- 页码：1557-1563
- 全文大小：903K
- 年卷期：v.83,no.5(March 1, 2011)
- ISSN：1520-6882
文摘
A method for the convenient detection of lead at the parts-per-billion (ppb)-level has been developed; it uses a conventional compact disc (CD) as the platform for preparing DNAzyme assays and an unmodified optical drive of ordinary desktop/laptop computers as the readout device. In particular, by immobilization of Pb2+-specific DNAzyme sensing constructs on the 鈥渢ransparent side鈥?of a conventional CD-R via mild surface reactions, the Pb2+ concentration can be determined by a free diagnostic program that checks the error distribution on the CD (i.e., it extracts the number of errors in a prerecorded audio file). The reading errors increase monotonically over a wide range of Pb2+ concentrations (from 10 nM to 1 mM), and the selectivity is confirmed by testing several other divalent cations (Zn2+, Ba2+, Mg2+, Ca2+, Cu2+, and Hg2+).