Fibronectin and Bone Morphogenetic Protein-2-Decorated Poly(OEGMA-r-HEMA) Brushes Promote Osseointegration of Titanium Surfaces

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• 作者：Xiaoshuai Ren ; Yuanzi Wu ; Yan Cheng ; Hongwei Ma ; Shicheng Wei
• 刊名：Langmuir
• 出版年：2011
• 出版时间：October 4, 2011
• 年：2011
• 卷：27
• 期：19
• 页码：12069-12073
• 全文大小：793K
• 年卷期：v.27,no.19(October 4, 2011)
• ISSN：1520-5827

文摘

To be better used as medical implants in orthopedic and dental clinical applications, titanium and titanium-based alloys need to be capable of inducing osteogenesis. Here we describe a method that allows the facile decoration of titanium surfaces to impart an osteogenesis capacity. A Ti surface was first deposited on a poly(OEGMA-r-HEMA) film using surface-initiated atom-transfer radical polymerization (SI-ATRP) with the further step of carboxylation. The modified surfaces were resistant to cell adhesion. Fibronectin (FN) and recombinant human bone morphogenetic protein-2 (rhBMP-2) were further immobilized onto p(OEGMA-r-HEMA) matrices. Our results demonstrate that the FN- and rhBMP-2-conjugated polymer surfaces could induce the adhesion of MC3T3 cells on Ti surfaces. Moreover, the protein-tethered surface exhibited enhanced cell differentiation in terms of alkaline phosphatase activity compared to that of the pristine Ti surface at similar cell proliferation rates. This research establishes a simple modification method of Ti surfaces via Ti-thiolate self-assembled monolayers (SAMs) and SI-ATRP and identifies a dual-functional Ti surface that combines antifouling and osseointegration promotion.