Gene Transfection of Mammalian Cells Using Membrane Sandwich Electroporation

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• 作者：Zhengzheng Fei ; Shengnian Wang ; Yubing Xie ; Brian E. Henslee ; Chee Guan Koh ; L. James Lee
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：August 1, 2007
• 年：2007
• 卷：79
• 期：15
• 页码：5719 - 5722
• 全文大小：368K
• 年卷期：v.79,no.15(August 1, 2007)
• ISSN：1520-6882

文摘

To avoid safety issues such as immune response andcytotoxicity associated with viruses and liposomes, physical methods have been widely used for either in vivo orex vivo gene delivery. They are, however, very invasive andoften provide limited efficiency. Using pEGFP and pSEAPplasmids and NIH 3T3 fibroblasts as models, we demonstrate a new electroporation-based gene delivery method,called membrane sandwich electroporation (MSE). TheMSE method is able to provide better gene confinementnear the cell surface to facilitate gene transport into thecells and thus shows significant improvement over transgene expression of mammalian cells compared to currentelectroporation techniques.