Aptamer-Based Plasmonic Sensor Array for Discrimination of Proteins and Cells with the Naked Eye

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• 作者：Yuexiang Lu ; Yueying Liu ; Suge Zhang ; Song Wang ; Sichun Zhang ; Xinrong Zhang
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：July 16, 2013
• 年：2013
• 卷：85
• 期：14
• 页码：6571-6574
• 全文大小：232K
• 年卷期：v.85,no.14(July 16, 2013)
• ISSN：1520-6882

文摘

We developed a colorimetric sensor array with reported protein aptamers as nonspecific receptors. We found that different target proteins could make the aptamer-protected gold nanoparticles (AuNPs) exhibit different aggregation behaviors in the presence of a high concentration salt and cause various color change. On the basis of this phenomenon, we applied a series of reported protein aptamers as a receptor array obtaining a distinct response pattern to each target protein. Seven proteins have been well distinguished with the naked eye at the 50 nM level. Cancerous human cells have also been discriminated from noncancerous cells. This method is simple, label-free, and sensitive. It will broaden the application filed of plasmonic nanoparticle-based sensors and give a new direction of developing sensitive array sensing systems.