Surface Modification for Enhancing Antibody Binding on Polymer-Based Microfluidic Device for Enzyme-Linked Immunosorbent Assay
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- 作者：Yunling Bai ; Chee Guan Koh ; Megan Boreman ; Yi-Je Juang ; I-Ching Tang ; L. James Lee ; Shang-Tian Yang
- 刊名：Langmuir
- 出版年：2006
- 出版时间：October 24, 2006
- 年：2006
- 卷：22
- 期：22
- 页码：9458 - 9467
- 全文大小：589K
- 年卷期：v.22,no.22(October 24, 2006)
- ISSN：1520-5827
文摘
A novel surface treatment method using poly(ethyleneimine) (PEI), an amine-bearing polymer, was developed toenhance antibody binding on the poly(methyl methacrylate) (PMMA) microfluidic immunoassay device. By treatingthe PMMA surface of the microchannel on the microfluidic device with PEI, 10 times more active antibodies canbe bound to the microchannel surface as compared to those without treatment or treated with the small amine-bearingmolecule, hexamethylenediamine (HMD). Consequently, PEI surface modification greatly improved the immunoassayperformance of the microfluidic device, making it more sensitive and reliable in the detection of IgG. The improvementcan be attributed to the spacer effect as well as the functional amine groups provided by the polymeric PEI molecules.Due to the smaller dimensions (140 × 125 
m) of the microchannel, the time required for antibody diffusion andadsorption onto the microchannel surface was reduced to only several minutes, which was 10 times faster than thesimilar process carried out in 96-well plates. The microchip also had a wider detection dynamic range, from 5 to 1000ng/mL, as compared to that of the microtiter plate (from 2 to 100 ng/mL). With the PEI surface modification, PMMA-based microchips can be effectively used for enzyme linked immunosorbent assays (ELISA) with a similar detectionlimit, but much less reagent consumption and shorter assay time as compared to the conventional 96-well plate.
m) of the microchannel, the time required for antibody diffusion andadsorption onto the microchannel surface was reduced to only several minutes, which was 10 times faster than thesimilar process carried out in 96-well plates. The microchip also had a wider detection dynamic range, from 5 to 1000ng/mL, as compared to that of the microtiter plate (from 2 to 100 ng/mL). With the PEI surface modification, PMMA-based microchips can be effectively used for enzyme linked immunosorbent assays (ELISA) with a similar detectionlimit, but much less reagent consumption and shorter assay time as compared to the conventional 96-well plate.