Quantitative Intracellular Molecular Profiling Using a One-Dimensional Flow System
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- 作者：Leiji Zhou ; Kemin Wang ; Weihong Tan ; Yunqing Chen ; Xinbing Zuo ; Jianhui Wen ; Bin Liu ; Hongxing Tang ; Lifang He ; Xiaohai Yang
- 刊名：Analytical Chemistry
- 出版年：2006
- 出版时间：September 1, 2006
- 年：2006
- 卷：78
- 期：17
- 页码：6246 - 6251
- 全文大小：369K
- 年卷期：v.78,no.17(September 1, 2006)
- ISSN：1520-6882
文摘
We report on the development of one-dimensional microfluidic bead arrays for rapid and quantitative molecularprofiling of human cancer cells. This new bioanalyticalplatform integrates the rapid binding kinetics of suspension bead carriers, the multiplexing and encoding capabilities of gene/protein chips, and the liquid handlingadvantages of microfluidic devices. Using antibody-conjugated beads in a two-site "sandwich" format, wedemonstrate that the proteomic contents of as few as 56human lung epithelial cancer cells can be determined withhigh sensitivity and specificity. The results indicate thateach cell contains ~6 × 105 copies of the tumor suppressor protein P53. We have further examined the expression changes of P53, c-Myc, and 
-Actin as a function ofanticancer drug treatment and have validated these changesby using Western blotting. This ability to quantitativelyanalyze normal and diseased cells raises new possibilitiesin studying cancer heterogeneity and circulating tumorcells.
-Actin as a function ofanticancer drug treatment and have validated these changesby using Western blotting. This ability to quantitativelyanalyze normal and diseased cells raises new possibilitiesin studying cancer heterogeneity and circulating tumorcells.