Extraction of Soybean Oil from Single Cells

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• 作者：Naoya Kasai ; Yusuke Imashiro ; and Naofumi Morita
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2003
• 出版时间：October 8, 2003
• 年：2003
• 卷：51
• 期：21
• 页码：6217 - 6222
• 全文大小：901K
• 年卷期：v.51,no.21(October 8, 2003)
• ISSN：1520-5118

文摘

Single cells prepared from autoclaved soybeans and cellulase treatment of the cells were effectivein digesting the cell walls of and extracting the oil from soybeans. The first cell wall of the soybeansingle cell was completely removed using cellulases; the thin and transparent second cell wall of thecell was swollen. Oil in the cell formed spherical or hemispherical oil drops, and oil leaking from theoil bodies was observed. The oil was almost retained within the second cell wall. Water-extractablesubstances were obtained at ~>60% of the weight. Flotation of oil drops by centrifugation was easilydone. Ambient n-hexane extraction was also possible; however, residual oil remained in the oil bodies.Protease or peptidase digested the structure of the oil bodies; however, separation of the oil and thehydrolysates was impossible. The oil from the oil bodies was obtained effectively (>85%) by pressingthe single cells and/or cellulase-treated single cells.Keywords: Soybean oil; oil extraction; cellulase; single cell