Two-in-One Strategy for Effective Enrichment of Phosphopeptides Using Magnetic Mesoporous 纬-Fe2O3 Nanocrystal Clusters

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• 作者：Yuting Zhang ; Lulu Li ; Wanfu Ma ; Ying Zhang ; Meng Yu ; Jia Guo ; Haojie Lu ; Changchun Wang
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2013
• 出版时间：February 13, 2013
• 年：2013
• 卷：5
• 期：3
• 页码：614-621
• 全文大小：433K
• 年卷期：v.5,no.3(February 13, 2013)
• ISSN：1944-8252

文摘

Designed with a two-in-one strategy, the magnetic mesoporous 纬-Fe₂O₃ nanocrystal clusters (m-纬-Fe₂O₃) have been successfully prepared for integrating the functions of effective enrichment and quick separation of phosphopeptides into a single architecture. First, the mesoporous Fe₃O₄ nanocrystal clusters (mFe₃O₄) were synthesized by solvothermal reaction and then were subjected to calcination in air to form m-纬-Fe₂O₃. The obtained m-纬-Fe₂O₃ have spherical morphology with uniform particle size of about 200 nm and mesoporous structure with the pore diameter of about 9.7 nm; the surface area is as large as 117.8 m²/g, and the pore volume is 0.34 cm³/g. The m-纬-Fe₂O₃ possessed very high magnetic responsiveness (Ms = 78.8 emu/g, magnetic separation time from solution is less than 5 s) and were used for the selective enrichment of phosphopeptides for the first time. The experimental results demonstrated that the m-纬-Fe₂O₃ possessed high selectivity for phosphopeptides at a low molar ratio of phosphopeptides/nonphosphopeptides (1:100), high sensitivity (the detection limit was at the fmol level), high enrichment recovery (as high as 89.4%), and excellent speed (the enrichment can be completed in 10 min). Moreover, this material is also quite effective for enrichment of phosphopeptides from the real sample (drinking milk), showing great potential in the practical application.

Keywords:

mesoporous 纬-Fe₂O₃; nanocrystal clusters; magnetic separation; phosphopeptides; enrichment