Bioactive Peptides/Chitosan Nanoparticles Enhance Cellular Antioxidant Activity of (鈭?-Epigallocatechin-3-gallate
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- 作者：Bing Hu ; Yuwen Ting ; Xiaoxiong Zeng ; Qingrong Huang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：January 30, 2013
- 年：2013
- 卷：61
- 期：4
- 页码：875-881
- 全文大小：328K
- 年卷期：v.61,no.4(January 30, 2013)
- ISSN：1520-5118
文摘
(鈭?-Epigallocatechin-3-gallate (EGCG), one representative of the well-studied chemopreventive phytochemicals but with low bioavailability, was encapsulated in monodispersed nanoparticles that were assembled from bioactive caseinophosphopeptide (CPP) and chitosan (CS). The encapsulation efficiency of EGCG in CS鈥揅PP nanoparticles ranged from 70.5 to 81.7%; meanwhile, the in vitro release of EGCG from CS鈥揅PP nanoparticles was in a controllable manner. The EGCG-loaded CS鈥揅PP nanoparticles exerted stronger activity of scavenging free radical than the free EGCG (p < 0.01) in the cellular antioxidant activity assay. Furthermore, cellular uptake of the EGCG-loaded CS鈥揅PP nanoparticles was confirmed by the green fluorescence inside the human hepatocellular caricinoma (HepG2) cells, which was considered to play an important role in the improvement of the antioxidant activity of the nanoencapsulated EGCG. The results suggested that encapsulation of EGCG using CS鈥揅PP nanoparticles should be a potential approach to enhance its antioxidant activity in biological systems.