Recent progress in metabolomics and the development of increasingly sensitive analytical techniques have renewed interest in global profi
ling, i.e., semiquantitative monitoring of all chemical constituents of biological fluids. In this work, we have performed global profi
ling of NIST SRM 1950, 鈥淢etabo
lites in Human Plasma鈥? using GC-MS, LC-MS, and NMR. Metabolome coverage, difficulties, and reproducibi
lity of the experiments on each platform are discussed. A total of 353 metabo
lites have been identified in this material. GC-MS provides 65 unique identifications, and most of the identifications from NMR overlap with the LC-MS identifications, except for some small sugars that are not directly found by LC-MS. Also, repeatabi
lity and intermediate precision analyses show that the SRM 1950 profi
ling is reproducible enough to consider this material as a good choice to distinguish between analytical and biological variabi
lity. C
linical laboratory data shows that most results are within the reference ranges for each assay. In-house computational tools have been developed or modified for MS data processing and interactive web display. All data and programs are freely available on
line at
http://peptide.nist.gov/ and http://srmd.nist.gov/.