Nongenetic Reprogramming of a Fungal Highly Reducing Polyketide Synthase

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• 作者：Ahmed A. Yakasai ; Jack Davison ; Zahida Wasil ; Laura M. Halo ; Craig P. Butts ; Colin M. Lazarus ; Andrew M. Bailey ; Thomas J. Simpson ; Russell J. Cox
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：July 20, 2011
• 年：2011
• 卷：133
• 期：28
• 页码：10990-10998
• 全文大小：1000K
• 年卷期：v.133,no.28(July 20, 2011)
• ISSN：1520-5126

文摘

The biosynthesis of the fungal metabolite tenellin from Beauveria bassiana CBS110.25 was investigated in the presence of the epigenetic modifiers 5-azacytidine and suberoyl bis-hydroxamic acid and under conditions where individual genes from the tenellin biosynthetic gene cluster were silenced. Numerous new compounds were synthesized, indicating that the normal predominant biosynthesis of tenellin is just one outcome out of a diverse array of possible products. The structures of the products reveal key clues about the programming selectivities of the tenellin polyketide synthase.