Quantitative Analysis of Gold Nanoparticles in Single Cells by Laser Ablation Inductively Coupled Plasma-Mass Spectrometry

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• 作者：Meng Wang ; Ling-Na Zheng ; Bing Wang ; Han-Qing Chen ; Yu-Liang Zhao ; Zhi-Fang Chai ; Helen J. Reid ; Barry L. Sharp ; Wei-Yue Feng
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：October 21, 2014
• 年：2014
• 卷：86
• 期：20
• 页码：10252-10256
• 全文大小：219K
• ISSN：1520-6882

文摘

Single cell analysis has become an important field of research in recent years reflecting the heterogeneity of cellular responses in biological systems. Here, we demonstrate a new method, based on laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS), which can quantify in situ gold nanoparticles (Au NPs) in single cells. Dried residues of picoliter droplets ejected by a commercial inkjet printer were used to simulate matrix-matched calibration standards. The gold mass in single cells exposed to 100 nM NIST Au NPs (Reference material 8012, 30 nm) for 4 h showed a log-normal distribution, ranging from 1.7 to 72 fg Au per cell, which approximately corresponds to 9 to 370 Au NPs per cell. The average result from 70 single cells (15 卤 13 fg Au per cell) was in good agreement with the result from an aqua regia digest solution of 1.2 脳 10⁶ cells (18 卤 1 fg Au per cell). The limit of quantification was 1.7 fg Au. This paper demonstrates the great potential of LA-ICPMS for single cell analysis and the beneficial study of biological responses to metal drugs or NPs at the single cell level.