Quantum Efficiency Modification of Organic Fluorophores Using Gold Nanoparticles on DNA Origami Scaffolds

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• 作者：Suchetan Pal ; Palash Dutta ; Haining Wang ; Zhengtao Deng ; Shengli Zou ; Hao Yan ; Yan Liu
• 刊名：The Journal of Physical Chemistry C
• 出版年：2013
• 出版时间：June 20, 2013
• 年：2013
• 卷：117
• 期：24
• 页码：12735-12744
• 全文大小：490K
• 年卷期：v.117,no.24(June 20, 2013)
• ISSN：1932-7455

文摘

We used DNA origami as a platform to coassemble a 20 nm gold nanoparticle (AuNP) and an organic fluorophore (TAMRA) and studied the distance-dependent plasmonic interactions between the particle and the dye using steady state fluorescence and lifetime measurements. Greater fluorescence quenching was found at smaller dye-particle distances, which was accompanied by an enhancement of the decay rate. We also fabricated 20 and 30 nm AuNP homodimers using DNA origami scaffolds and positioned a Cy3 fluorophore between the AuNPs in both assemblies. For each particle size, three different interparticle distances were investigated. Up to 50% enhancement of the Cy3 fluorescence quantum efficiency was observed for the dye between the 30 nm AuNPs. These results are in good agreement with the theoretical simulations.