The roots of two varieties of
Polygonum cuspidatum (Hu Zhang and Mexican Bamboo) were analyzedfor resveratrol and analogues. The roots of each variety were dried and ground into a powder. Thepowdered roots were then extracted with methanol and ethyl acetate. The ethyl acetate fraction ofthe Mexican Bamboo was then subjected to fractionation and purification using silica gel columnchromatography and semipreparative HPLC. In addition to resveratrol (3,5,4'-trihydroxystilbene),three stilbene glucosides were identified by
1H NMR,
13C NMR, and MS. The stilbene glucosideswere shown to be a piceatannol glucoside (3,5,3',4'-tetrahydroxystilbene 4'-
O-
-
D-glucopyranoside),resveratroloside (3,5,4'-trihydroxystilbene 4'-
O-
-
D-glucopyranoside), and piceid (3,5,4'-trihydroxystilbene 3-
O-
-
D-glucopyranoside). The levels of the piceatannol glucoside and piceid were twice ashigh in the Mexican Bamboo as compared to the Hu Zhang.Keywords:
Polygonum cuspidatum; stilbene glucoside; resveratrol; piceatannol; resveratroloside;piceid; Hu Zhang; Mexican Bamboo; Japanese Knotweed