Biomimetically Ornamented Rapid Prototyping Fabrication of an Apatite鈥揅ollagen鈥揚olycaprolactone Composite Construct with Nano鈥揗icro鈥揗acro Hierarchical Structure for Large Bone Defect Treatment
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- 作者：Jinbing Wang ; Dingyu Wu ; Zhanzhao Zhang ; Jun Li ; Yi Shen ; Zhenxing Wang ; Yu Li ; Zhi-Yong Zhang ; Jian Sun
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2015
- 出版时间：December 2, 2015
- 年：2015
- 卷：7
- 期：47
- 页码：26244-26256
- 全文大小：939K
- ISSN：1944-8252
文摘
Biomaterial-based bone graft substitute with favorable mechanical and biological properties could be used as an alternative to autograft for large defect treatment. Here, an apatite鈥揷ollagen鈥損olycaprolactone (Ap-Col-PCL) composite construct was developed with unique nano鈥搈icro鈥搈acro hierarchical architectures by combining rapid prototyping (RP) fabrication technology and a 3D functionalization strategy. Macroporous PCL framework was fabricated using RP technology, then functionalized by collagen incorporation and biomimetic deposition. Ap-Col-PCL composite construct was characterized with hierarchical architectures of a nanoscale (鈭?00 nm thickness and 鈭? 渭m length) platelike apatite coating on the microporous (126 卤 18 渭m) collagen networks, which homogeneously filled the macroporous (鈭?000 渭m) PCL frameworks and possessed a favorable hydrophilic property and compressive modulus (68.75 卤 3.39 MPa) similar to that of cancellous bone. Moreover, in vitro cell culture assay and in vivo critical-sized bone defect implantation demonstrated that the Ap-Col-PCL construct could not only significantly increase the cell adhesion capability (2.0-fold) and promote faster cell proliferation but also successfully bridge the segmental long bone defect within 12 weeks with much more bone regeneration (5.2-fold), better osteointegration (7.2-fold), and a faster new bone deposition rate (2.9-fold). Our study demonstrated that biomimetically ornamented Ap-Col-PCL constructs exhibit a favorable mechanical property, more bone tissue ingrowth, and better osteointegration capability as an effective bone graft substitute for critical-sized bone defect treatment; meanwhile, it can also harness the advantages of RP technology, in particular, facilitating the customization of the shape and size of implants according to medical images during clinical application.