文摘
The effect of different fertilizers on the 未15N value, nitrate concentration, and nitrate reductase activity of Brassica campestris and the 未15N value of soil has been investigated through a pot experiment. The 未15N mean value of B. campestris at the seedling stage observed in the composted chicken treatment (+8.65鈥? was higher than that of chemical fertilizer treatment (+5.73鈥?, compost-chemical fertilizer (+7.53鈥?, and control check treatment (+7.86鈥?. There were significantly different 未15N values (p < 0.05) between B. campestris cultivated with composted chicken manure treatment and with chemical fertilizer treatment. The similar results were also found at the middle stage and the terminal stage. The variation of 未15N value in soil for different treatments was smaller than that of B. campestris, which was +6.71鈥?8.12鈥? +6.83鈥?8.24鈥? and +6.85鈥?.4鈥? respectively, at seedling stage, middle stage, and terminal stage. With the growth of B. campestris, the nitrate content decreased in all treatments, and the nitrate reductase activity in B. campestris increased except for the CK. Results suggested that the 未15N values of B. campestris and soil were more effected by the fertilizer than by the dose level, and the 未15N value analysis could be used as a tool to discriminate the B. campestris cultivated with composted manure or chemical fertilizer.
Keywords:
stable nitrogen isotope; fertilizer; nitrate; nitrate reductase; Brassica campestris