A Laminar Flow Electroporation System for Efficient DNA and siRNA Delivery

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• 作者：Zewen Wei ; Deyao Zhao ; Xueming Li ; Mengxi Wu ; Wei Wang ; Huang Huang ; Xiaoxia Wang ; Quan Du ; Zicai Liang ; Zhihong Li
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：August 1, 2011
• 年：2011
• 卷：83
• 期：15
• 页码：5881-5887
• 全文大小：975K
• 年卷期：v.83,no.15(August 1, 2011)
• ISSN：1520-6882

文摘

By introducing a hydrodynamic mechanism into a microfluidics-based electroporation system, we developed a novel laminar flow electroporation system with high performance. The laminar buffer flow implemented in the system separated the cell suspension flow from the electrodes, thereby excluding many unfavorable effects due to electrode reaction during electroporation, such as hydrolysis, bubble formation, pH change, and heating. Compared to conventional microfluidic electroporation systems, these improvements significantly enhanced transfection efficiency and cell viability. Furthermore, successful electrotransfection of plasmid DNA and, more importantly, synthetic siRNA, was demonstrated in several hard-to-transfect cell types using this system.