Plain Silver Surface Plasmon Resonance for Microarray Application

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• 作者：Zhiqiang Cheng ; Zhiyou Wang ; Doreen E. Gillespie ; Christopher Lausted ; Zheng Zheng ; Mo Yang ; Jinsong Zhu
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：February 3, 2015
• 年：2015
• 卷：87
• 期：3
• 页码：1466-1469
• 全文大小：330K
• ISSN：1520-6882

文摘

The application scope of surface plasmon resonance (SPR) and SPR imaging (SPRi) is rapidly growing, and tools such as high-performance and low-cost slides could enable more rapid growth of the field. We describe herein a novel silver slide, addressing the inherent instability of plain silver structure by improving adhesion between the glass substrate and the silver layer with a thin buffer layer of gold. Covered by a self-assembled monolayer (SAM) only, SPR characteristics of the slide remain steady for more than 3 months under regular storage. In a bioassay, the slide substantiates the predicted nearly 100% sensitivity improvement over gold slides and exhibits exceptional performance stability as determined by sensitivity and resolution measurements during the extended 40鈥?00 s multicycle experiment. We demonstrate the suitability of this new slide for large-area SPRi, describing analysis results from a 1鈥?96-ligand protein microarray. We predict this slide structure will provide a stable, high-sensitivity solution for high-throughput SPRi applications and other surface analysis platforms.