Integrated Lectin Affinity Microfluidic Chip for Glycoform Separation

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• 作者：Xiuli Mao ; Yong Luo ; Zhongpeng Dai ; Keyi Wang ; Yuguang Du ; and Bingcheng Lin
• 刊名：Analytical Chemistry
• 出版年：2004
• 出版时间：December 1, 2004
• 年：2004
• 卷：76
• 期：23
• 页码：6941 - 6947
• 全文大小：202K
• 年卷期：v.76,no.23(December 1, 2004)
• ISSN：1520-6882

文摘

Lectin affinity chromatography was miniaturized into amicrofluidic format, which results in improvement ofperformance, as compared to the conventional method.A lectin affinity monolith column was prepared in themicrochannel of a microfluidic chip. The porous monolithwas fabricated by UV-initiated polymerization of ethylenedimethacrylate (EDMA) and glycidyl methacrylate (GMA)in the presence of porogeneities, followed by immobilization of pisum sativum agglutinin (PSA) on the monolithmatrix. Using electroosmosis as the driven force, lectinaffinity chromatographies of three kinds of glycoprotein,turkey ovalbumin (TO), chicken ovalbumin (CO), andovomucoid (OM), were carried out on the microfluidicsystem. All the glycoproteins were successfully separatedinto several fractions with different affinities toward theimmobilized PSA. The integrated system reduces the timerequired for the lectin affinity chromatography reactionto ~3%, thus, the overall analysis time from 4 h to 400 s.Only 300 pg of glycoprotein is required for the wholeseparation process. Moreover, troublesome operations forlectin affinity chromatography are simplified.