RGD-Conjugated Copolymer Incorporated into Composite of Poly(lactide-co-glycotide) and Poly(l-lactide)-Grafted Nanohydroxyapatite for Bone Tissue Engineering
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- 作者：Peibiao Zhang ; Haitao Wu ; Han Wu ; Zhongwen Lu虁 ; Chao Deng ; Zhongkui Hong ; Xiabin Jing ; Xuesi Chen
- 刊名：Biomacromolecules
- 出版年：2011
- 出版时间：July 11, 2011
- 年：2011
- 卷：12
- 期：7
- 页码：2667-2680
- 全文大小：1711K
- 年卷期：v.12,no.7(July 11, 2011)
- ISSN：1526-4602
文摘
Various surface modification methods of RGD (Arg-Gly-Asp) peptides on biomaterials have been developed to improve cell adhesion. This study aimed to examine a RGD-conjugated copolymer RGD/MPEG-PLA-PBLG (RGD-copolymer) for its ability to promote bone regeneration by mixing it with the composite of poly(lactide-co-glycotide) (PLGA) and hydroxyapatite nanoparticles surface-grafted with poly(l-lactide) (g-HAP). The porous scaffolds were prepared using solvent casting/particulate leaching method and grafted to repair the rabbit radius defects after seeding with autologous bone marrow mesenchymal cells (MSCs) of rabbits. After incorporation of RGD-copolymer, there were no significant influences on scaffold鈥檚 porosity and pore size. Nitrogen of RGD peptide, and calcium and phosphor of g-HAP could be exposed on the surface of the scaffold simultaneously. Although the cell viability of its leaching liquid was 92% that was lower than g-HAP/PLGA, its cell adhesion and growth of 3T3 and osteoblasts were promoted significantly. The greatest increment in cell adhesion ratios (131.2鈥?57.1% higher than g-HAP/PLGA) was observed when its contents were 0.1鈥? wt % but only at 0.5 h after cell seeding. All the defects repaired with the implants were bridged after 24 weeks postsurgery, but the RGD-copolymer contained composite had larger new bone formation and better fusion interface. The composites containing RGD-copolymer enhanced bone ingrowth but presented more woven bones than others. The combined application of RGD-copolymer and bone morphological protein 2 (BMP-2) exhibited the best bone healing quality and was recommended as an optimal strategy for the use of RGD peptides.