Dual Enzyme-like Activities of Iron Oxide Nanoparticles and Their Implication for Diminishing Cytotoxicity
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- 作者：Zhongwen Chen ; Jun-Jie Yin ; Yu-Ting Zhou ; Yu Zhang ; Lina Song ; Mengjie Song ; Sunling Hu ; Ning Gu
- 刊名：ACS Nano
- 出版年：2012
- 出版时间：May 22, 2012
- 年：2012
- 卷：6
- 期：5
- 页码：4001-4012
- 全文大小：715K
- 年卷期：v.6,no.5(May 22, 2012)
- ISSN：1936-086X
文摘
Iron oxide nanoparticles (IONPs) are frequently used in biomedical applications, yet their toxic potential is still a major concern. While most studies of biosafety focus on cellular responses after exposure to nanomaterials, little is reported to analyze reactions on the surface of nanoparticles as a source of cytotoxicity. Here we report that different intracellular microenvironment in which IONPs are located leads to contradictive outcomes in their abilities to produce free radicals. We first verified pH-dependent peroxidase-like and catalase-like activities of IONPs and investigated how they interact with hydrogen peroxide (H2O2) within cells. Results showed that IONPs had a concentration-dependent cytotoxicity on human glioma U251 cells, and they could enhance H2O2-induced cell damage dramatically. By conducting electron spin resonance spectroscopy experiments, we showed that both Fe3O4 and 纬-Fe2O3 nanoparticles could catalyze H2O2 to produce hydroxyl radicals in acidic lysosome mimic conditions, with relative potency Fe3O4 > 纬-Fe2O3, which was consistent with their peroxidase-like activities. However, no hydroxyl radicals were observed in neutral cytosol mimic conditions with both nanoparticles. Instead, they decomposed H2O2 into H2O and O2 directly in this condition through catalase-like activities. Transmission electron micrographs revealed that IONPs located in lysosomes in cells, the acidic environment of which may contribute to hydroxyl radical production. This is the first study regarding cytotoxicity based on their enzyme-like activities. Since H2O2 is continuously produced in cells, our data indicate that lysosome-escaped strategy for IONP delivery would be an efficient way to diminish long-term toxic potential.