Integrin-Targeted Trifunctional Probe for Cancer Cells: A 鈥淪eeing and Counting鈥?Approach

详细信息    查看全文

• 作者：Zhubao Zhang ; Qiang Luo ; Xiaowen Yan ; Zhaoxin Li ; Yacui Luo ; Limin Yang ; Bo Zhang ; Haifeng Chen ; Qiuquan Wang
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：November 6, 2012
• 年：2012
• 卷：84
• 期：21
• 页码：8946-8951
• 全文大小：352K
• 年卷期：v.84,no.21(November 6, 2012)
• ISSN：1520-6882

文摘

We report the design and synthesis of a trifunctional probe for seeing and counting cancer cells using both fluorescence imaging (FI) and inductively coupled plasma mass spectrometry (ICPMS) for the first time. It consisted of a guiding cyclic RGD peptide unit to catch cancer cells via targeting the 伪_v尾₃ integrin overexpressed on their surface, a 5-amino-fluorescein moiety for FI using confocal laser scanning microscopy (CLSM) as well as a 2-aminoethyl-monoamide-DOTA group for loading stable europium ion and subsequent ICPMS quantification of the cancer cells without the use of radioactive isotopes. In addition to FI, the LOD (3蟽) of the 伪_v尾₃ integrin was down to 69.2鈥?09.4 amol per cell depending on the type of the 伪_v尾₃-positive cancer cells when using ICPMS and those of the cancer cell number reached 17鈥?5. This probe developed enables us not only to see but also to count the 伪_v尾₃-positive cancer cells ultrasensitively, paving a new way for early diagnosis of cancer.