Solution-State 2D NMR Spectroscopy of Plant Cell Walls Enabled by a Dimethylsulfoxide-d6/1-Ethyl-3-methylimidazolium Acetate Solvent

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• 作者：Kun Cheng ; Hagit Sorek ; Herbert Zimmermann ; David E. Wemmer ; Markus Pauly
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：March 19, 2013
• 年：2013
• 卷：85
• 期：6
• 页码：3213-3221
• 全文大小：468K
• 年卷期：v.85,no.6(March 19, 2013)
• ISSN：1520-6882

文摘

Lignocellulosic biomass is composed of the polysaccharides cellulose and hemicellulose and the polyphenol lignin. Many current methods for analyzing the structure of lignocelluloses involve a sequential extraction of the material and subsequent analysis of the resulting fractions, which is labor-intensive and time-consuming. The work presented here assesses the dissolution of whole lignocellulosic material, focusing on biomass derived from the perennial bioenergy grass Miscanthus. The solvent dimethylsulfoxide (DMSO)-d₆ containing 1-ethyl-3-methylimidazolium acetate ([Emim]OAc) was able to dissolve lignocellulosic material completely and gave high-resolution 2D heteronuclear single quantum coherence (HSQC) NMR spectra of the entire array of wall polymers. Extrapolated time-zero HSQC was applied using DMSO-d₆/[Emim]OAc-d₁₄ and enabled quantitative analysis of structural traits of lignocellulose components.