Profiling the Membrane and Glycosaminoglycan-Binding Proteomes of Moraxella catarrhalis

详细信息    查看全文

• 作者：Anita Karner ; Bernd Gesslbauer ; Anton Spreitzer ; Johannes Almer ; Margarita Smidt ; Wolfgang Schüler ; Berthold Fartmann ; Wolfgang Zimmermann ; Andreas Meinke ; Andreas J. Kungl
• 刊名：Journal of Proteome Research
• 出版年：2016
• 出版时间：September 2, 2016
• 年：2016
• 卷：15
• 期：9
• 页码：3055-3097
• 全文大小：530K
• 年卷期：0
• ISSN：1535-3907

文摘

Moraxella catarrhalis, a Gram-negative bacterium, is an important respiratory pathogen causing acute otitis media and exacerbations of chronic obstructive pulmonary disease. Adhesion of the pathogen to human epithelial cells is mediated via bacterial membrane adhesin proteins. To identify the surface proteome of Moraxella catarrhalis, we applied different membrane protein extraction methods in combination with different proteomic technologies. Proteins from preparations of outer membrane vesicles and from carbonate extractions were analyzed using either a gel-based nano-HPLC–MS/MS technique or 2D-LC–MS/MS. Furthermore, because glycosaminoglycans (GAGs) play an important role for microbial entry into human cells, the GAG-binding membranome of Moraxella catarrhalis was investigated using a glycan-based pull-down approach. By these means, potential vaccine protein candidates that were previously selected by the ANTIGENome technology were confirmed, but importantly also novel proteins were identified as candidates.