Efficient Solid-Phase Chemical Synthesis of 5′-Triphosphates of DNA, RNA, and their Analogues

详细信息    查看全文

• 作者：Ivan Zlatev ; Thomas Lavergne ; Franoise Debart ; Jean-Jacques Vasseur ; Muthiah Manoharan ; Franois Morvan
• 刊名：Organic Letters
• 出版年：2010
• 出版时间：May 21, 2010
• 年：2010
• 卷：12
• 期：10
• 页码：2190-2193
• 全文大小：196K
• 年卷期：v.12,no.10(May 21, 2010)
• ISSN：1523-7052

文摘

A robust, reproducible, and scalable method for the solid-phase synthesis of 5′-triphosphates of DNA, RNA, and their chemically modified analogues using 5′-H-phosphonate intermediates is described. 5′-Triphosphates of oligonucleotides with varying lengths and sequences containing different 5′-terminal nucleotides, with and without internal sugar-backbone modifications, were efficiently prepared as crude products or further purified by HPLC.