Comparative Metabolic Profiling Reveals Secondary Metabolites Correlated with Soybean Salt Tolerance

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• 作者：Wei Wu ; Qing Zhang ; Yanming Zhu ; Hon-Ming Lam ; Zongwei Cai ; Dianjing Guo
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：December 10, 2008
• 年：2008
• 卷：56
• 期：23
• 页码：11132-11138
• 全文大小：267K
• 年卷期：v.56,no.23(December 10, 2008)
• ISSN：1520-5118

文摘

High-performance liquid chromatography−ultraviolet−electrospray ionization mass spectrometry (HPLC-UV-ESI-MS) and HPLC-ESI-MSⁿ analysis methods were used for metabolic profiling and simultaneous identification of isoflavonoids and saponins in soybean seeds. Comparative targeted metabolic profiling revealed marked differences in the metabolite composition between salt-sensitive and salt-tolerant soybean varieties. Principle component analysis clearly demonstrated that it is possible to use secondary metabolites, for example, isoflavones and saponins, to discriminate between closely related soybean genotypes. Genistin and group B saponins were identified as the key secondary metabolites correlated with salt tolerance. These individual metabolites may provide additional insight into the salt tolerance and adaptation of plants.