Spectroelectrochemical Examination of the Interaction between Bacterial Cells and Gold Electrodes

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• 作者：Juan Pablo Busalmen ; Antonio Bern&aacute ; Juan Miguel Feliu
• 刊名：Langmuir
• 出版年：2007
• 出版时间：May 22, 2007
• 年：2007
• 卷：23
• 期：11
• 页码：6459 - 6466
• 全文大小：298K
• 年卷期：v.23,no.11(May 22, 2007)
• ISSN：1520-5827

文摘

The interaction between bacterial cells of Pseudomonas fluorescens (ATCC 17552) and gold electrodes was analyzedby cyclic voltammetry (CV) and attenuated total reflection-surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). The voltammetric evaluation of cell adsorption showed a decrease in the double-layer capacitance ofpolyoriented single-crystal gold electrodes with cell adhesion. As followed by IR spectroscopy in the ATR configuration,the adsorption of bacterial cells onto thin-film gold electrodes was mainly indicated by the increase in intensity withtime of amide I and amide II protein-related bands at 1664 and 1549 cm^-1, respectively. Bands at 1448 and 2900cm^-1 corresponding to the scissoring and the stretching bands of CH₂ were also detected, together with a minor peakat 1407 cm^-1 due to the v_s COO^- stretching. Weak signals at 1237 cm^-1 were due to amide III, and a broad bandbetween 1100 and 1200 cm^-1 indicated the presence of alcohol groups. Bacteria were found to displace water moleculesand anions coadsorbed on the surface in order to interact with the electrode intimately. This fact was evidenced inthe SEIRAS spectra by the negative features appearing at 3450 and 3575 cm^-1, corresponding to interfacial waterdirectly interacting with the electrode and water associated with chloride ions adsorbed on the electrode, respectively.Experiments in deuterated water confirmed these assignments and allowed a better estimation of amide absorptionbands. In CV experiments, an oxidation process was observed at potentials higher than 0.4 V that was dependent onthe exposure time of electrodes in concentrated bacterial suspensions. Adsorbed bacterial cells were found to get closerto the gold surface during oxidation, as indicated by the concomitant increment in the main IR bacterial signalsincluding amide I, a sharp band at 1240 cm^-1, and a broad one at 1120 cm^-1 related to phosphate groups in the bacterialmembranes. It is proposed to be due to the oxidation of lipopolysaccharides on the outermost bacterial surface.