Single Molecule Ionic Current Sensing in Segmented Flow Microfluidics

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• 作者：Thomas R. Gibb ; Aleksandar P. Ivanov ; Joshua B. Edel ; Tim Albrecht
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：February 4, 2014
• 年：2014
• 卷：86
• 期：3
• 页码：1864-1871
• 全文大小：427K
• ISSN：1520-6882

文摘

Herein, we describe the integration of two glass nanopores into a segmented flow microfluidic device with a view on enhancing the functionality of label free, single molecule nanopore sensors. Within a robust and mechanically stable platform, individual droplet compositions are distinguished before single molecule translocations from the droplet are detected electrochemically via the Coulter principle. This result is highly significant, combining the sensitivity of single molecule methods and their ability to overcome the clouding of the ensemble average with the 鈥渋solated microreactor鈥?benefits of droplet microfluidics. Furthermore, devices as presented here provide the platform for the development of systems where the injection and extraction of single molecules allow droplet composition to be controlled at the molecular level.