Toxicity of Hydrolyzed Vicilins toward Callosobruchus maculatus and Phytopathogenic Fungi
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- 作者：Adriana Ferreira Ucha ; Maria Raquel Alcntara de Miranda ; Amanda Jardim de Souza ; Valdirene Moreira Gomes ; Ktia Valevski Sales Fernandes ; Francisco Jos Alves Lemos ; Antonia Elenir Amancio Olivei
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2009
- 出版时间：September 9, 2009
- 年：2009
- 卷：57
- 期：17
- 页码：8056-8061
- 全文大小：804K
- 年卷期：v.57,no.17(September 9, 2009)
- ISSN：1520-5118
文摘
Studies have shown that vicilins (7S storage proteins) from seeds were able to bind to the surface of the Callosobruchus maculatus larval midgut and to the peritrophic matrices of the midguts of Diatraea saccharalis and Tenebrio molitor, inhibiting larval development. Vicilins were also shown to inhibit yeast growth and bind to yeast cells through the association with chitin-containing structures. The present work studies the association of peptides from vicilins of genotypes of Vigna unguiculata (susceptible and resistant to bruchid) with acetylated chitin and the toxicity of vicilin fragments and chitin-binding vicilin fragments to C. maculatus and phytopathogenic fungi. Hydrolysis of vicilins with α-chymotrypsin results in a complex mixture of fragments that were separated by chitin-affinity chromatography. Chitin-binding peptides from both genotypes were toxic to C. maculatus larvae, and α-chymotrypsin-hydrolyzed vicilins were deleterious to the above insect and to Fusarium oxysporum, Colletotrichum musae, and Saccharomyces cerevisiae fungi.