Electrochemical Sensor for Lead Cation Sensitized with a DNA Functionalized Porphyrinic Metal鈥揙rganic Framework
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- 作者：Lin Cui ; Jie Wu ; Jie Li ; Huangxian Ju
- 刊名：Analytical Chemistry
- 出版年：2015
- 出版时间：October 20, 2015
- 年：2015
- 卷：87
- 期：20
- 页码：10635-10641
- 全文大小：430K
- ISSN：1520-6882
文摘
An efficient electrochemical sensor was presented for lead cation detection using a DNA functionalized iron鈥損orphyrinic metal鈥搊rganic framework (GR鈥?/(Fe鈥揚)n-MOF) as a probe. The newly designed probe showed both the recognition behavior of GR鈥? to Pb2+ with high selectivity and the excellent mimic peroxidase performance of (Fe鈥揚)n-MOF. In the presence of Pb2+, GR鈥? could be specifically cleaved at the ribonucleotide (rA) site, which produced the short (Fe鈥揚)n-MOF-linked oligonucleotide fragment to hybridize with hairpin DNA immobilized on the surface of screen-printed carbon electrode (SPCE). Because of the mimic peroxidase property of (Fe鈥揚)n-MOF, enzymatically amplified electrochemical signal was obtained to offer the sensitive detection of Pb2+ ranging from 0.05 to 200 nM with a detection limit of 0.034 nM. In addition, benefiting from the Pb2+-dependent GR鈥?, the proposed assay could selectively detect Pb2+ in the presence of other metal ions. The SPCE based electrochemical sensor along with the GR鈥?/(Fe鈥揚)n-MOF probe exhibited the advantages of low-cost, simple fabrication, high sensitivity and selectivity, providing potential application of on-site and real-time Pb2+ detection in complex media.