A Direct, Efficient Method for the Preparation of siRNAs Containing Ribo-like North Bicyclo[3.1.0]hexane Pseudosugars

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• 作者：Montserrat Terrazas ; Anna Avin虄o&#769 ; Maqbool A. Siddiqui ; Victor E. Marquez ; Ramon Eritja
• 刊名：Organic Letters
• 出版年：2011
• 出版时间：June 3, 2011
• 年：2011
• 卷：13
• 期：11
• 页码：2888-2891
• 全文大小：750K
• 年卷期：v.13,no.11(June 3, 2011)
• ISSN：1523-7052

文摘

An efficient method for the preparation of siRNAs modified with ribo-like North bicyclo[3.1.0]hexane pseudosugars is described. The combined use of 2鈥?O-(2-cyanoethoxymethyl) (CEM) and 2鈥?O-TBDMS protection was successfully employed for RNA synthesis with the added advantage that both groups were efficiently removed in a single step. The resulting North ribo-methanocarba-modified siRNAs are compatible with the intracellular RNAi machinery and can mediate specific degradation of target mRNA.