Unique, Polyfucosylated Glycan–Receptor Interactions Are Essential for Regeneration of Hydra magnipapillata

详细信息    查看全文

• 作者：Sonu Sahadevan ; Aristotelis Antonopoulos ; Stuart M. Haslam ; Anne Dell ; Subramanian Ramaswamy ; Ponnusamy Babu
• 刊名：ACS Chemical Biology
• 出版年：2014
• 出版时间：January 17, 2014
• 年：2014
• 卷：9
• 期：1
• 页码：147-155
• 全文大小：410K
• ISSN：1554-8937

文摘

Cell鈥揷ell communications, cell鈥搈atrix interactions, and cell migrations play a major role in regeneration. However, little is known about the molecular players involved in these critical events, especially cell surface molecules. Here, we demonstrate the role of specific glycan鈥搑eceptor interactions in the regenerative process using Hydra magnipapillata as a model system. Global characterization of the N- and O-glycans expressed by H. magnipapillata using ultrasensitive mass spectrometry revealed mainly polyfucosylated LacdiNAc antennary structures. Affinity purification showed that a putative C-type lectin (accession number Q6SIX6) is a likely endogenous receptor for the novel polyfucosylated glycans. Disruption of glycan鈥搑eceptor interactions led to complete shutdown of the regeneration machinery in live Hydra. A time-dependent, lack-of-regeneration phenotype observed upon incubation with exogenous fuco-lectins suggests the involvement of a polyfucose receptor-mediated signaling mechanism during regeneration. Thus, for the first time, the results presented here provide direct evidence for the role of polyfucosylated glycan鈥搑eceptor interactions in the regeneration of H. magnipapillata.