Carbon-14 Biolabeling of (+)-Catechin and Proanthocyanidin Oligomers in Willow Tree Cuttings
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- 作者：Sté ; phanie Dé ; prez ; Isabelle Mila ; and Augustin Scalbert
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：1999
- 出版时间：October 1999
- 年：1999
- 卷：47
- 期：10
- 页码：4219 - 4230
- 全文大小：187K
- 年卷期：v.47,no.10(October 1999)
- ISSN：1520-5118
文摘
Proanthocyanidin polymers, oligomers, and the structurally related monomer (+)-catechin werelabeled by incorporation of radioactive precursors in shoots of willow tree (Salix caprea L.). [1-14C]-Acetate and [U-14C]-phenylalanine precursors were fed through the cut stems or petioles of leaves.Optimization of several parameters such as the nature and origin of the plant material, leaf maturity,nature, and quantity of radioactive precursor applied and the duration of metabolism led toincorporation yields of 3.2% and to specific activities of 500 
Ci/g. Detailed characterization of theproducts (polymerization degree, procyanidin/prodelphinidin ratio, specific activities) and purificationby chromatography are reported. Some sugars bound to radiolabeled proanthocyanidin polymerswere removed by enzymic treatment with a mixture of glycosidases. A radioactive purity close to100% and specific activities suitable for bioavailability studies were obtained.Keywords: Proanthocyanidins; Salix caprea; willow tree; biosynthesis; radiolabeling; bioavailability
Ci/g. Detailed characterization of theproducts (polymerization degree, procyanidin/prodelphinidin ratio, specific activities) and purificationby chromatography are reported. Some sugars bound to radiolabeled proanthocyanidin polymerswere removed by enzymic treatment with a mixture of glycosidases. A radioactive purity close to100% and specific activities suitable for bioavailability studies were obtained.Keywords: Proanthocyanidins; Salix caprea; willow tree; biosynthesis; radiolabeling; bioavailability