Isolation, Antifungal Activity, and Structure Elucidation of the Glutarimide Antibiotic, Streptimidone, Produced by Micromonospora coerulea
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- 作者：Beom Seok Kim ; Surk Sik Moon ; and Byung Kook Hwang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：1999
- 出版时间：August 1999
- 年：1999
- 卷：47
- 期：8
- 页码：3372 - 3380
- 全文大小：100K
- 年卷期：v.47,no.8(August 1999)
- ISSN：1520-5118
文摘
The antibiotic Ao58A,which showed strong antifungal activity against some plant pathogenic fungi,was purified from the culture broth and mycelial mats of Micromonospora coerulea strain Ao58using various chromatographic procedures. The molecular formula of the antibiotic Ao58A wasdeduced to be C16H23NO4 (M + H, m/z 294.1707) by high-resolution FAB mass spectroscopy. Analysesof 1H NMR, 13C NMR, and 2D NMR spectral data revealed that the antibiotic Ao58A is theglutarimide antibiotic streptimidone, 4-(2-hydroxy-5,7-dimethyl-4-oxo-6,8-nonadienyl)-2,6-piperidinedione. The antibiotic Ao58A was very effective in inhibiting growth of Phytophthora capsici,Didymella bryoniae, Magnaporthe grisea, and Botrytis cinerea in the range ~3-10 
g mL-1 of MICs.In vivo evaluation of the antibiotic Ao58A under greenhouse condition showed strong control efficaciesagainst the development of P. capsici, B. cinerea, and M. grisea on pepper, cucumber, and rice plants,respectively. The antibiotic Ao58A was equally as effective as metalaxyl, vinclozolin, and tricyclazolein the control of these plant diseases. However, it did not show any phytotoxicity on the plantseven when treated with 500 g mL-1.Keywords: Micromonospora coerulea; streptimidone; antifungal activity; plant disease control
g mL-1 of MICs.In vivo evaluation of the antibiotic Ao58A under greenhouse condition showed strong control efficaciesagainst the development of P. capsici, B. cinerea, and M. grisea on pepper, cucumber, and rice plants,respectively. The antibiotic Ao58A was equally as effective as metalaxyl, vinclozolin, and tricyclazolein the control of these plant diseases. However, it did not show any phytotoxicity on the plantseven when treated with 500 g mL-1.Keywords: Micromonospora coerulea; streptimidone; antifungal activity; plant disease control