Selecting Aptamers for a Glycoprotein through the Incorporation of the Boronic Acid Moiety
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- 作者：Minyong Li ; Na Lin ; Zhen Huang ; Lupei Du ; Craig Altier ; Hao Fang ; Binghe Wang
- 刊名：Journal of the American Chemical Society
- 出版年：2008
- 出版时间：September 24, 2008
- 年：2008
- 卷：130
- 期：38
- 页码：12636-12638
- 全文大小：180K
- 年卷期：v.130,no.38(September 24, 2008)
- ISSN：1520-5126
文摘
The first general method for the selection of boronic acid−based aptamers (boronolectins) that allows for glycan substructure focusing is described. Using fibrinogen as a model glycoprotein, we have selected boronic acid-modified DNA aptamers that have high affinities (low nM Kd) and the ability to recognize changes in the glycosylation site. The method developed should also be applicable to the development of aptamers for other glycoproducts, such as glycolipids and glycopeptides.