Toward Larger DNA Origami
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- 作者：Alexandria N. Marchi ; Ishtiaq Saaem ; Briana N. Vogen ; Stanley Brown ; Thomas H. LaBean
- 刊名：Nano Letters
- 出版年：2014
- 出版时间：October 8, 2014
- 年：2014
- 卷：14
- 期：10
- 页码：5740-5747
- 全文大小：466K
- ISSN：1530-6992
文摘
Structural DNA nanotechnology, and specifically scaffolded DNA origami, is rapidly developing as a versatile method for bottom-up fabrication of novel nanometer-scale materials and devices. However, lengths of conventional single-stranded scaffolds, for example, 7,249-nucleotide circular genomic DNA from the M13mp18 phage, limit the scales of these uniquely addressable structures. Additionally, increasing DNA origami size generates the cost burden of increased staple-strand synthesis. We addressed this 2-fold problem by developing the following methods: (1) production of the largest to-date biologically derived single-stranded scaffold using a 位/M13 hybrid virus to produce a 51鈥?66-nucleotide DNA in a circular, single-stranded form and (2) inexpensive DNA synthesis via an inkjet-printing process on a chip embossed with functionalized micropillars made from cyclic olefin copolymer. We have experimentally demonstrated very efficient assembly of a 51-kilobasepair origami from the 位/M13 hybrid scaffold folded by chip-derived staple strands. In addition, we have demonstrated two-dimensional, asymmetric origami sheets with controlled global curvature such that they land on a substrate in predictable orientations that have been verified by atomic force microscopy.