Measurement of Large Distances in Biomolecules Using Double-Quantum Filtered Refocused Electron Spin-Echoes
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- 作者：Peter P. Borbat ; Jared H. Davis ; Samuel E. Butcher ; and Jack H. Freed
- 刊名：Journal of the American Chemical Society
- 出版年：2004
- 出版时间：June 30, 2004
- 年：2004
- 卷：126
- 期：25
- 页码：7746 - 7747
- 全文大小：80K
- 年卷期：v.126,no.25(June 30, 2004)
- ISSN：1520-5126
文摘
It is shown how the new technique of double-quantum filtered refocused electron spin-echoes is a significant improvement over double-quantum coherence ESR, since it increases the experimental acquisition time. This enables the measurement of longer distances in bilabeled biomolecules. The method is demonstrated on a long double-stranded A-type RNA, spin labeled at both ends. The measured distance of 72 Å is in excellent agreement with molecular modeling.