Temperature Sensing Using Fluorescent Nanothermometers

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• 作者：Fiorenzo Vetrone ; Rafik Naccache ; Alicia Zamarrón ; Angeles Juarranz de la Fuente ; Francisco Sanz-Rodríguez ; Laura Martinez Maestro ; Emma Martín Rodriguez ; Daniel Jaque ; José García Solé ; John A. Capobianco
• 刊名：ACS Nano
• 出版年：2010
• 出版时间：June 22, 2010
• 年：2010
• 卷：4
• 期：6
• 页码：3254-3258
• 全文大小：260K
• 年卷期：0
• ISSN：1936-086X

文摘

Acquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive fluorescence of NaYF₄:Er³⁺,Yb³⁺ nanoparticles, where the intensity ratio of the green fluorescence bands of the Er³⁺ dopant ions (²H_11/2 → ⁴I_15/2 and ⁴S_3/2 → ⁴I_15/2) changes with temperature. The nanothermometers were first used to obtain thermal profiles created when heating a colloidal solution of NaYF₄:Er³⁺,Yb³⁺ nanoparticles in water using a pump−probe experiment. Following incubation of the nanoparticles with HeLa cervical cancer cells and their subsequent uptake, the fluorescent nanothermometers measured the internal temperature of the living cell from 25 °C to its thermally induced death at 45 °C.