General Concept of High-Performance Amperometric Detector for Microfluidic (Bio)Analytical Chips

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• 作者：Christian Amatore ; Nicolas Da Mota ; Catherine Sella ; Laurent Thouin
• 刊名：Analytical Chemistry
• 出版年：2008
• 出版时间：July 1, 2008
• 年：2008
• 卷：80
• 期：13
• 页码：4976 - 4985
• 全文大小：1200K
• 年卷期：v.80,no.13(July 1, 2008)
• ISSN：1520-6882

文摘

In this work, we established theoretically that amperometric detector arrays consisting of a series of parallel band microelectrodes placed on the wall of a microchannel may offer excellent analytical detection performances when implemented onto microfluidic (bio)analytical devices after the separative stages. In combination with the concentration imprinting strategies reported in a previous work, these exceptional performances may be extended to nonelectroactive or poorly diffusing analytes. Using an array of electrodes instead of a large single band allows the whole core of the channel to be probed though keeping an excellent time resolution. Thus, analytes with close retention times may be characterized individually with a resolution which eventually outpaces that of spectroscopic detections. Such important advantages may be obtained only through a complete understanding of the complex coupling between diffusional and convective transport of molecules in microfluidic solutions near an electrochemical detector. As a consequence, the conditions underlying the theoretical data presented in this work have been selected after optimizing procedures rooted on previous theoretical analyses. They will be fully disclosed in a series of further works that will also establish the experimental performances of such amperometric detectors and validate the present concept.