Resolving Isomeric Peptide Mixtures: A Combined HPLC/Ion Mobility-TOFMS Analysis of a 4000-Component Combinatorial Library

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• 作者：Catherine A. Srebalus Barnes ; Amy E. Hilderbrand ; Stephen J. Valentine ; and David E. Clemmer
• 刊名：Analytical Chemistry
• 出版年：2002
• 出版时间：January 1, 2002
• 年：2002
• 卷：74
• 期：1
• 页码：26 - 36
• 全文大小：287K
• 年卷期：v.74,no.1(January 1, 2002)
• ISSN：1520-6882

文摘

A reversed-phase high-performance liquid chromatography (HPLC) separation approach has been combined withion mobility/time-of-flight (TOF) mass spectrometry inorder to characterize a combinatorial peptide librarydesigned to contain 4000 peptides of the general formNH₂-Xxx-Xxx-Xxx-CO₂H, NH₂-Ala-Xxx-Xxx-Xxx-CO₂H, NH₂-Ser-Ala-Xxx-Xxx-Xxx-CO₂H and NH₂-Leu-Ser-Ala-Xxx-Xxx-Xxx-CO₂H (where Xxx represents a randomization over10 different amino acids: Ala, Arg, Asp, Glu, Gly, Leu,Lys, Phe, Ser, and Val). Addition of the gas-phase mobilityseparation between the HPLC separation and TOF measurement dimensions makes it possible to resolve manypeptide isomers that have identical retention times (andmasses).